Project information
Application of adsorptive transfer and elemination techniques in oligonucleotides and nucleic acids analysis.
- Project Identification
- IAA1163201
- Project Period
- 1/2002 - 12/2004
- Investor / Pogramme / Project type
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Academy of Sciences of the Czech Republic
- Grants of distinctly investigative character focused on the sphere of research pursued at present particularly in the ASCR
- MU Faculty or unit
- Faculty of Science
- Keywords
- Oligonucleotides; nucleic acids; adsorptive transfer technique; elimination voltammetry nith linear
- Cooperating Organization
-
Institute of Biophysics
- Responsible person RNDr. František Jelen, CSc.
The principal aims of the joint research are (a) to develop new ways of detecting of nucleic acids bases using transfer technique in connection with elimination and stripping voltammetry, (b) to study short oligonucleotides in dependence on their sequence and length and (c) to use elimination voltammetry with linear scan and electrochemical impedance spectroscopy in oligonucleotides and nucleic acids analysis. Analysis will be performed at mercury and solid electrodes. It is expected that the results obtained in this project will be utilized as a simple sensitive and selective analytical procedures for the determination of nucleic acids and their components. The advatage of these analytical procedures consists in the possibility to modify electrodes by nucleic acids and oligonucleotides. Obtained results will be applied in various fields of biophysics, biochemistry and molecular biology.
Publications
Total number of publications: 52
2004
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Determination of azidothymidine - an antiproliferative and virostatic drug by square wave voltammetry
Electroanalysis, year: 2004, volume: 2004, edition: 16
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Determination of isoflavones in soybean food and human urine using liquid chromatography with electrochemical detection
Journal of Chromatography B, year: 2004, volume: 806, edition: 1
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Determination of isoflavones using high-performance liquid chromatography with electrochemical detection (HPLC- ED)
Book of Abstracts - 55th Annual Meeting of the ISE, year: 2004
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Electroanalytical determination of d(GCGAAGC) hairpin
Bioelectrochemistry, year: 2004, volume: 2004, edition: 63
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Electrochemical determination of cysteine-containing biomolecules by means of catalytic reactions on a mercury electrode
Book of Abstracts - 55th Annual Meeting of the ISE, year: 2004
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Electrochemical determination of lead and glutathione in a plant cell culture
Bioelectrochemistry, year: 2004, volume: 2004, edition: 63
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Elektrochemické stanovení těžkých kovů ve složité biologické matrici
Sborník abstrakt IV. pracovní setkání fyzikálních chemiků a elektrochemiků, year: 2004
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Elimination voltammetry of oligonucleotides
Book of Abstracts I - 55th Annual Meeting of the ISE, year: 2004
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IV. Pracovní setkání fyzikálních chemiků a elektrochemiků
Year: 2004, type: Conference
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Square wave and elimination voltammetric analysis of azidothymidine in the presence of oligonucleotides and chromosomal DNA
Bioelectrochemistry, year: 2004, volume: 2004, edition: 63