Project information
Early diagnostics of leukemia using DNA-microchip technology and by monitoring of epigenetic changes
- Project Identification
- GP301/04/P136
- Project Period
- 1/2004 - 12/2006
- Investor / Pogramme / Project type
-
Czech Science Foundation
- Postdoctoral projects
- MU Faculty or unit
- Faculty of Informatics
- Keywords
- DNA-microchipes, leukemia, epigenetic changes, chromatin structure
Using DNA-microchip technology expression maps in cells of bone marrow or immobilized blood of patients with different types of leukaemia at different stages will be obtained and compared with results obtained from cells without effect of cancer. The differences found by comparing expression maps of cells with various level of progression and differentiation will help us to the identify primary, yet known, but also new genes whose false function contributes to the disease development.
Also it should be clarified how the changes of activity of these genes reflect the activity of other known or unknown genes and what is the location of these genes on corresponding chromosomes. For epigenetic changes FISH and high-resolution cytometry will be used. Chromosomes with modulated level of expression will be interrogated structural-epigenetic-study. Changes in structure of chromatin in the cells of patients with leukaemia in the various stages of diseases in contrast to non-cancer cells will be evaluated
Results
Publications
Total number of publications: 13
2007
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New insights into gene positional clustering and its properties supported by large-scale analysis of various differentiation pathways
Genomics, year: 2007, volume: 89, edition: 1
2006
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Evaluation of CD34+ - and Lin- -selected cells from peripheral blood stem cell grafts of patients with lymphoma during differentiation in culture ex vivo using a cDNA microarray technique
Experimental hematology, year: 2006, volume: 34, edition: 7
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Transcription density map as a new tool for evaluation of various expression profiles: Ex vivo differentiation of CD34+ cells
Year: 2006, type: Conference abstract
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Vybrané aplikace technologie cDNA microarrays v onkologickém výzkumu
Klinická onkologie, year: 2006, volume: 19, edition: 2
2005
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Analýza obrazů získaných snímáním microarrays
Year: 2005, type: Conference abstract
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Aplikace FDR a FWER testovacích procedur na data ze studie kolorektálního karcinomu
Year: 2005, type: Conference abstract
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Combination of cDNA microarray technology and fluorescence leads to better understanding of relationships between gene expression and epigenetic regulation
Year: 2005, type: Conference abstract
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Důkaz klastrování genů na chromosomových doménách studiem diferenciací buněčných nádorových linií
Year: 2005, type: Conference abstract
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New practical tools for microarray data analysis
Year: 2005, type: Conference abstract
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Normalizační metody ve zpracování dat z DNA Microarrays
Year: 2005, type: Conference abstract