Project information
Foton-upkonverzní značky pro mikrofluidní jednomolekulové imunostanovení proteinových biomarkerů
- Project Identification
- GA21-03156S
- Project Period
- 4/2021 - 9/2024
- Investor / Pogramme / Project type
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Czech Science Foundation
- Standard Projects
- MU Faculty or unit
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Faculty of Science
- doc. Mgr. Zdeněk Farka, Ph.D.
- doc. Mgr. Pavel Bouchal, Ph.D.
- Mgr. Pavla Bouchalová, Ph.D.
- Mgr. Matěj Pastucha, Ph.D.
- prof. RNDr. Petr Skládal, CSc.
- Keywords
- photon-upconversion; nanoparticles; immunochemistry; biomarkers; microfluidics; single-molecule methods
- Cooperating Organization
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Institute of Analytical Chemistry of the ASCR, v. v. i.
- Responsible person Mgr. Antonín Hlaváček, Ph.D.
The project proposes a new development of single-molecule immunoassays using the luminescence of photon-upconversion nanoparticles. This type of luminescence is characterized by excitation in the infrared region and emission of short-wavelength radiation (mostly visible). The low background of this type of luminescence simplifies the imaging and counting of individual nanoparticles; when used as a luminescent label, the counting of the single analyte molecules is possible. The innovation is based on using microfluidic chips for automation. Microfluidics decreases analysis time, reagent consumption, detection limits, and facilitates the analysis of microscopic samples (single-cell analysis). The methodology will be verified by the detection of known protein biomarkers: (A) prostate-specific antigen in blood serum and plasma and (B) protein biomarker p53 at the level of individual cells. The project aims to improve disease diagnostics and monitoring the course of disease treatment and advances the toolbox of cell and molecular biology.
Sustainable Development Goals
Masaryk University is committed to the UN Sustainable Development Goals, which aim to improve the conditions and quality of life on our planet by 2030.
Publications
Total number of publications: 32
2022
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Detekce proteinových biomarkerů pomocí lateral flow testů s foton-upkonverzními nanočásticemi
Czech Chem. Soc. Symp. Ser. 20, 335–339 (2022), year: 2022
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Immunomagnetic Assay Using Upconversion Nanoparticles for the Detection of Prostate-Specific Antigen
Year: 2022, type: Conference abstract
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Influence of Label and Solid Support on the Performance of Heterogeneous Immunoassays
Analytical Chemistry, year: 2022, volume: 94, edition: 47, DOI
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Lateral flow immunoassay based on photon-upconversion nanoparticles for the detection of protein biomarkers
Year: 2022, type: Conference abstract
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Phage-mediated bacterial lysis studied by AFM and SPR
Year: 2022, type: Appeared in Conference without Proceedings
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PMVEMA-coated upconverting nanoparticles for upconversion-linked immunoassay of cardiac troponin
Talanta, year: 2022, volume: 244, edition: 123400, DOI
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Up and down the spectrum: upconversion nanocrystal and semiconductor material fused into a single nanocomposite
Light: Science & Applications, year: 2022, volume: 11, edition: 179, DOI
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Upconversion immunoassay with magnetic separation for the detection of cancer biomarkers
Year: 2022, type: Conference abstract
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Upconversion-linked immunoassays (ULISA) for food and environmental monitoring and single-molecule (digital) diagnostic assays
Year: 2022, type: Conference abstract