Role of PCNA and TLS polymerases in D-loop extension during homologous recombination in humans
| Authors | |
|---|---|
| Year of publication | 2013 |
| Type | Article in Periodical |
| Magazine / Source | DNA Repair |
| MU Faculty or unit | |
| Citation | |
| Doi | http://dx.doi.org/10.1016/j.dnarep.2013.05.001 |
| Field | Genetics and molecular biology |
| Keywords | TLS polymerases; Homologous recombination; DNA repair synthesis; D-loop; Reconstitution |
| Description | Homologous recombination (HR) is essential for maintaining genomic integrity, which is challenged by a wide variety of potentially lethal DNA lesions. Regardless of the damage type, recombination is known to proceed by RAD51-mediated D-loop formation, followed by DNA repair synthesis. Nevertheless, the participating polymerases and extension mechanism are not well characterized. Here, we present a reconstitution of this step using purified human proteins. In addition to Pol delta, TLS polymerases, including Pol eta and Pol kappa, also can extend D-loops. In vivo characterization reveals that Pol eta and Pol kappa are involved in redundant pathways for HR. In addition, the presence of PCNA on the D-loop regulates the length of the extension tracks by recruiting various polymerases and might present a regulatory point for the various recombination outcomes. |
| Related projects: |