Analysis of Plant Meiotic Chromosomes by Chromosome Painting

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Authors	LYSÁK Martin MANDÁKOVÁ Terezie
Year of publication	2013
Type	Chapter of a book
MU Faculty or unit	Central European Institute of Technology
Citation
Description	Chromosome painting (CP) refers to visualization of large chromosome regions, entire chromosome arms, or entire chromosomes via fluorescence in situ hybridization (FISH). For CP in plants, contigs of chromosome-specific bacterial artificial chromosomes (BAC) from the target species or from a closely related species (comparative chromosome painting, CCP) are typically applied as painting probes. Extended pachytene chromosomes provide the highest resolution of CP in plants. CP enables identification and tracing of particular chromosome regions and/or entire chromosomes throughout all meiotic stages as well as corresponding chromosome territories in premeiotic interphase nuclei. Meiotic pairing and structural chromosome rearrangements (typically inversions and translocations) can be identified by CP. Here, we describe step-by-step protocols of CP and CCP in plant species including chromosome preparation, BAC DNA labeling, and multicolor FISH.
Related projects:	Chromosome evolution in crucifers (Brassicaceae) revealed by comparative chromosome painting Evoluce genomu alopolyploidních řeřišnic (Cardamine) rozdílného fylogenetického stáří