Permanent Genetic Resources added to Molecular Ecology Resources Database 1 April 2013-31 May 2013
Authors | |
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Year of publication | 2013 |
Type | Article in Periodical |
Magazine / Source | Molecular Ecology Resources |
MU Faculty or unit | |
Citation | |
Doi | http://dx.doi.org/10.1111/1755-0998.12140 |
Field | Genetics and molecular biology |
Keywords | microsatellites 454 GS-FLX Titanium sequencing cyprinids Barbus cross-amplification hybrid zones |
Description | Using cost-effective 454 GS-FLX Titanium sequencing technology, microsatellite markers were isolated de novo for Barbus meridionalis. Five multiplex sets including 28 newly and two previously-developed loci were optimized using allopatric populations of both B. barbus and B. meridionalis. Our study revealed the high success rate of cross-species amplification in two Balkan species, B. cyclolepis and B. balcanicus. |
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