Use of nanomaterials for TOF mass spectrometry of complex biomolecules and for tissue profiling
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| Year of publication | 2014 |
| Type | Conference abstract |
| MU Faculty or unit | |
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| Description | Mass spectrometry (MS) of complex biomolecules is mostly performed using Matrix-Assisted Laser Desorption Ionization (MALDI) with Time of Flight (TOF) detection. Development of nanotechnology and nanomaterials (NMs) and nanoparticles (NPs) with their unique physical and chemical properties brought to MS alternative media for efficient ionization of analytes and accelerated development of Surface-Assisted Laser Desorption Ionization (SALDI), Surface-Enhanced Laser Desorption Ionization (SELDI) and Nano-Assisted Laser Desorption Ionization (NALDI). These techniques contributed significantly to MS [1]. NMs possess also extensive applications in MALDI TOF MS tissue imaging in current nanomedicine [2]. In this work, we studied applications of various NMs (either as matrices or surfaces) in MS analysis of histological tissue sections. We also evaluated effect of NMs on the ionization of various peptides and the comparison with common organic matrices was done. LDI of biomolecules from different metallic and/or nano-surfaces documented that NPs increase the ionization, improve homogeneity of samples and reduce the background of salts as compared with conventional matrices. Our data documented that NMs increase signal intensity in mass spectra of various peptides but also of very complex tissue samples. NMs supported MALDI-TOF MS thus represent efficient technique advancing global approaches, such as tissue proteomics. |
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