Benzofurazane as a New Redox Label for Electrochemical Detection of DNA: Towards Multipotential Redox Coding of DNA Bases

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Authors	BALINTOVÁ Jana PLUCNARA Medard VIDLÁKOVÁ Pavlína POHL Radek HAVRAN Luděk FOJTA Miroslav HOCEK Michal
Year of publication	2013
Type	Article in Periodical
Magazine / Source	Chemistry - A European Journal
MU Faculty or unit	Central European Institute of Technology
Citation
Web	http://onlinelibrary.wiley.com/doi/10.1002/chem.201301868/abstract;jsessionid=EF409FC4C3D959BEA050AC3B14DB1BA6.f01t04
Doi	http://dx.doi.org/10.1002/chem.201301868
Field	Biochemistry
Keywords	DNA polymerase; electrochemistry; nucleoside triphosphates; sequencing; voltammetry
Description	Benzofurazane has been attached to nucleosides and dNTPs, either directly or through an acetylene linker, as a new redox label for electrochemical analysis of nucleotide sequences. Primer extension incorporation of the benzofurazane-modified dNTPs by polymerases has been developed for the construction of labeled oligonucleotide probes. The combination of benzofurazane and nitrophenyl reducible labels has proved to be excellent for ratiometric analysis of nucleotide sequences and is suitable for bioanalytical applications.
Related projects:	Centrum nových přístupů k bioanalýze a molekulární diagnostice