Electrochemical behaviour of 2,4-dinitrophenylhydrazi(o)ne asmulti-redox centre DNA label at mercury meniscus modified silversolid amalgam electrode

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Authors	DAŇHEL Aleš RAINDLOVA Veronika HAVRAN Luděk PIVOŇKOVÁ Hana HOCEK Michal FOJTA Miroslav
Year of publication	2014
Type	Article in Periodical
Magazine / Source	Electrochimica Acta
MU Faculty or unit	Central European Institute of Technology
Citation
Web	http://dx.doi.org/10.1016/j.electacta.2013.09.147
Doi	http://dx.doi.org/10.1016/j.electacta.2013.09.147
Field	Electrochemistry
Keywords	DNA; Redox labeling; 2_4-Dinitrophenylhydrazine; Silver solid amalgam electrode; Voltammetry
Description	A detailed voltammetric study of 2,4-dinitrophenylhydrazine (DNPH), a DNPH modified nucleotide(dCDNPHMP) and a short single stranded DNA (ssDNA-DNPH) at mercury meniscus modified silver solid amalgam electrode (m-AgSAE) is presented in this work. Electrochemical behaviour of these compounds was studied using cyclic voltammetry (CV), adsorptive stripping CV and open circuit adsorptive transfer stripping CV. Successful incorpo-ration of the DNPH labeled nucleoside triphosphate into the DNA structure shows that selected DNApolymerase tolerates this bulky moiety and can be used, in combination with electrochemical methodsutilizing m-AgSAE, in further development of various applications.
Related projects:	Centrum nových přístupů k bioanalýze a molekulární diagnostice