Development of new agamococcidia Rhytidocystis sp. from Travisia forbesii
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| Year of publication | 2014 |
| Type | Conference abstract |
| MU Faculty or unit | |
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| Description | Agamococcidia (Apicomplexa) represent a small and poorly investigated group, inhabiting digestive tract of marine polychaetes and Hexacorallia. They have an unusual life cycle lacking gametogony and merogony. This study focuses on light and electron microscopic investigation of parasite from marine polychaete Travisia forbesii. In intestine, intracellular stages of Rhythidocystis sp. were observed. Stages appeared as white dots of various shape and size. Free cells are oval or round, immotile. Under TEM, we observed putative sporozoites, several stages of growing trophozoites, paired associations and presumably zygotes. Sporozoites were of oval shape and localized in cytoplasm of entherocytes. They were covered by a trimembrane pellicle; cytoplasm was packed with several mitochondria, endoplasmic reticulum. Maturing trophozoites were oval, possessed cytoplasm filled with lipid droplets, protein inclusions, amylopectin granules and round to oval nucleus. Further stages oval to round exhibited, darker cytoplasm filled with the same set of inclusions and more condense MIT. Last one stage was at least two times larger than previous ones. Its pellicle formed numerous invaginations. These structures resembled short folds arranged in rosette-like pattern. Numerous micropore openings were located on the surface of cell. The micropores could be found in all stages. The cytoplasm was packed with reserve granules, Golgi bodies, MIT, ER and lobed nucleus. Paired associations were also observed. Cells considered to be zygotes possessed dark cytoplasm with amylopectin granules and electron-dense vacuoles. We assume that sporozoite penetrates into the epithelial cell, and during Th maturation it moves to the base of intestinal epithelium cell and induces syncytium formation and, than other bud the sporoblast that transform into sporozoites, other form zygote. We acknowledge the WSBS of MSU and financial support from ECIP – GAČR No. GBP505/12/G112. |
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