DETERMINATION OF TESTOSTERONE AND ITS 5 METABOLITES IN THE INCUBATION MEDIA AS A MEASURE OF CYTOCHROME P450 METABOLIC ACTIVITY
| Authors | |
|---|---|
| Year of publication | 2014 |
| Type | Article in Proceedings |
| Conference | CECE 2014: 11TH INTERNATIONAL INTERDISCIPLINARY MEETING ON BIOANALYSIS |
| MU Faculty or unit | |
| Citation | |
| Field | Biochemistry |
| Keywords | LIQUID-CHROMATOGRAPHY |
| Description | The innovative HPLC method for the determination of testosterone and its five metabolites in incubation medium with rat liver microsomes is reported. The method utilizes liquid-liquid extraction procedure, with use of internal standard prednisone. Analytes are eluted within 18.7 min. The method enables reliable determination of testosterone, 7 alpha-hydroxytestosterone, 6 beta-hydroxytestosterone, 16 alpha-hydroxytestosterone, 16 beta-hydroxytestosterone, 2 beta-hydroxytestosterone with LOD in the range 1.9-3.9 nmol/L. |
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