Thiamine Metabolism Alterations in Gestational Diabetes Complicated Pregnancy
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| Year of publication | 2014 |
| Type | Appeared in Conference without Proceedings |
| Citation | |
| Description | Thiamine is involved in the regulation of glucose metabolism. Thiamine is delivered to the cell via specific thiamine transporters 1 (THTR1 and 2 (THTR2). Within the cell thiamine is activated by enzyme thiamine pyrophosphokinase (TPK1). Transketolase (TKT) is the rate-limiting enzyme of non-oxidative branch of pentose phosphate pathway whose activity depends on thiamine diphosphate (TDP) as a cofactor. We aimed to determine plasma thiamine levels, levels of TDP in erythrocytes, erythrocyte TKT activity and thiamine effect in pregnant women with and without GDM. A total of 181 pregnant women were included in the study. Of those 104 had GDM, 77 had physiologic pregnancy. No significant difference in plasma thiamine levels between GDM and physiologic pregnancy group was found in pregnancy and after delivery (both P ? 0.05, Mann-Whitney test). Plasma thiamine levels in pregnancy were significantly lower than postpartum in both GDM and physiologic pregnancy group (P < 0.001 and P = 0.017, Wilcoxon test). Erythrocyte TDP in pregnancy was significantly higher in GDM patients compared to physiologic pregnancy group (P < 0.001, Mann-Whitney), however it did not differ postpartum (P ? 0.05, Mann-Whitney). Mid trimester erythrocyte TDP levels were significantly higher compared to postpartum in GDM (P < 0.001, Wilcoxon test) as well as in physiologic pregnancy group (P < 0.001, Wilcoxon paired test). Both GDM complicated and physiologic pregnancy are characterized by increase in erythrocyte TDP accompanied by parallel decrease in plasma thiamine during second trimester compared to postpartum. GDM is associated with significantly increased level of active form of thiamine (TDP) in erythrocytes during second trimester compared to physiologic pregnancy. However, TDP levels are normalised postpartum. |
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