Detection of parasitic helminths in final meat products
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| Year of publication | 2014 |
| Type | Appeared in Conference without Proceedings |
| MU Faculty or unit | |
| Citation | |
| Description | The changes in farming practices toward to bio-production, globalised food market with increasing transportation of animals and also global climate change facilitate the widespread dissemination of food-borne diseases, including the pathogens such as zoonotic multicellular parasites. These facts consequently induce the improvement of various diagnostic methods of food-borne agents in the final products such as meat. Our work is in this context focused on development of reliable comprehensive molecular diagnostic method useful for rapid control of final meat products designated for European and Czech market. We adopted high sensitive multiplex oligonucleotide ligation-PCR technique (MOL-PCR), representing the novel diagnostic platform based on magnetic microspheres and detection realized via instrument for qualitative and quantitative analysis (MAGPIX). This modern approach enables simultaneous direct screen of complex samples potentially containing the DNA from number of different parasitic organisms. Up to now, the molecular probes allowing the detection of targeted DNA, the further amplification of this probes and capturing system for generated products were designed for two parasitic worms - Trichinella spiralis (partial sequence of 18S rRNA gene) and Taenia saginata (partial sequence of COX1 gene). The calibration of the system and optimization of method is in process. |
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