Kinetic Study OF Ketamine N-Demethylation Mediated by On-Line Enantioselective Capillary Electrophoresis Method

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Authors	ŘEMÍNEK Roman GLATZ Zdeněk THORMANN Wolfgang
Year of publication	2014
Type	Article in Proceedings
Conference	11 th International Interdisciplinary Meeting on Bioanalysis CECE 2014
MU Faculty or unit	Faculty of Science
Citation
Field	Biochemistry
Keywords	Kapilární elektroforéza; Enantioselektivní separace; Cytochrom P450; CYP3A4; Ketamin
Description	Enantioselective discrimination is an important phenomenon influencing pharmacokinetics and pharmacodynamics of chiral drugs in humans and animals. Metabolism appraisal of single enantiomers of every drug candidate compound thus constitutes the inseparable part of LADME/tox screening. Capillary electrophoresis represents a promising technique in the field of in vitro enzyme assays due to miniscule sample consumption, simple implementation of chiral separations and high-throughput by automation. An optimized and economical capillary electrophoretic method for on-line studies of the enantioselective drug metabolism mediated by cytochrome P450 3A4 enzyme (CYP3A4) is introduced.
Related projects:	Miniaturized on-line drug metabolism system based on capillary electrophoresis