On-capillary derivatisation as an approach to enhancing sensitivity in capillary electrophoresis
| Authors | |
|---|---|
| Year of publication | 2015 |
| Type | Article in Periodical |
| Magazine / Source | Electrophoresis |
| MU Faculty or unit | |
| Citation | |
| Doi | http://dx.doi.org/10.1002/elps.201400449 |
| Field | Biochemistry |
| Keywords | capillary electrophoresis; derivatisation; enhancing of sensitivity; on-capillary derivatisation; |
| Description | Separation technologies play an important role in revealing biological processes at various omic levels, in pharmacological and clinical research. In this context capillary electrophoresis (CE) is a strong candidate for analyses of samples with rapidly increasing complexity. Even though CE is well known for its many advantages in this regard, the sensitivity of CE analyses is insufficient for many applications. Accordingly, there are generally three main options for enhancing the sensitivity of CE analyses – using special detection techniques, using sample preconcentration and derivatisation. Derivatisation is often the method of choice for many laboratories, since it is simple and provides several advantages such as small sample volume demand and the possibility of automation. |
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