Candida parapsilosis Biofilm Identification by Raman Spectroscopy

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Authors	SAMEK Ota MLYNÁRIKOVÁ Katarína BERNATOVÁ Silvie JEŽEK Jan KRZYŽÁNEK Vladislav ŠILER Martin ZEMÁNEK Pavel RŮŽIČKA Filip HOLÁ Veronika MAHELOVÁ Martina
Year of publication	2014
Type	Article in Periodical
Magazine / Source	International Journal of Molecular Sciences
MU Faculty or unit	Faculty of Medicine
Citation
Doi	http://dx.doi.org/10.3390/ijms151223924
Field	Microbiology, virology
Keywords	Raman spectroscopy; Candida parapsilosis; biofilm
Description	Colonies of Candida parapsilosis on culture plates were probed directly in situ using Raman spectroscopy for rapid identification of specific strains separated by a given time intervals (up to months apart). To classify the Raman spectra, data analysis was performed using the approach of principal component analysis (PCA). The analysis of the data sets generated during the scans of individual colonies reveals that despite the inhomogeneity of the biological samples unambiguous associations to individual strains (two biofilm-positive and two biofilm-negative) could be made.
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