Characterization of Enterococcus species occuring on terrestrial vegetation
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| Year of publication | 2014 |
| Type | Appeared in Conference without Proceedings |
| MU Faculty or unit | |
| Citation | |
| Description | A group of 173 presumptive plant-associated enterococcal strains was isolated during the vegetation periods in 2009 and 2013 in the frame of two studies dealing with the investigation of Enterococcus spp. populations occurring on plants. Individual strains of Gram-positive cocci were isolated on Kanamycin esculin azide agar and characterized using rep-PCR fingerprinting with the (GTG)5 primer. Obtained fingerprints were compared with an in-house CCM reference database containing more that 7000 fingerprints representing multiple Gram-positive species including representatives of all hitherto described Enterococcus spp. In total, 102 isolates matched reference database entries and were assigned as Enterococcus faecalis (23 strains), Enterococcus haemoperoxidus (20), Enterococcus plantarum (14), Enterococcus casseliflavus (12), Enterococcus faecium (9), Enterococcus ureilyticus (8), Enterococcus moraviensis (6), Enterococcus rotai (6), Enterococcus mundtii (2), and Enterococcus durans (2). Remaining 71 strains were identified as members of other genera (e.g. Lactococcus, Leuconostoc) or were not clustered with any fingerprints included in the database. Overall results obtained in the frame of the present work revealed that plants are inhabited by diverse and taxonomically rich Enterococcus spp. populations. This work was supported by the project CZ.1.07/2.3.00/20.0183. |
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