Determination of serum total protein by the Kjeldahl method
| Authors | |
|---|---|
| Year of publication | 2015 |
| Type | Article in Proceedings |
| Conference | Študentská vedecká konferencia PriF UK 2015 |
| MU Faculty or unit | |
| Citation | |
| Web | http://2015.svkprifuk.info/zbornik2015.pdf |
| Field | Analytic chemistry |
| Keywords | total protein; kjeldahl method; biuret method |
| Description | Chromý et al. found in 2009, that albumin-calibrated analysis of serum total protein according to a candidate reference method, developed by Doumas working group in 1981, provide results with unacceptable positive bias. The simplest way to rectify this defect is the use of serum and/or plasma-based protein standards certified by the Kjeldahl method. We reviewed Kjeldahl methods adopted by laboratory medicine and selected for total protein a direct analysis on washed protein precipitates with trichloroacetic acid and verified its preliminary analytical performance parameters. We describe a final simplified analysis with modified still unit and digestion flask serving for both digestion and distillation and present its re-validation. |
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