Immobilized cytochrome P450 2C9 microreactor: a promising tool in hits' and leads' screening
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| Year of publication | 2015 |
| Type | Conference abstract |
| Citation | |
| Description | In this work we present an immobilized enzyme microreactor (IMER) based on CYP 2C9 isoform, this IMER was integrated into capillary which further improves its features due to miniscule sample consumption and enabling rapid quantification by the means of capillary electrophoresis. Using this instrument a kinetic and inhibition study was performed while all kinetic and inhibition parameters were acquired and showed good correlation with literature. On the other hand a broadly used model for determination of the reactants’ effective concentration, the Hagen-Poiseuille law, showed some major discrepancies and therefore an adjusted model for concentration profile modelling is presented as well. |
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