Species assignment in Pupilla (Gastropoda: Pulmonata: Pupillidae): integration of DNA-sequence data and conchology
| Authors | |
|---|---|
| Year of publication | 2015 |
| Type | Article in Periodical |
| Magazine / Source | Journal of Molluscan Studies |
| MU Faculty or unit | |
| Citation | |
| Doi | http://dx.doi.org/10.1093/mollus/eyu083 |
| Field | Zoology |
| Keywords | Molluscs; Pupilla; phylogeny; taxonomy |
| Description | Using the Pupilla faunas of Europe, North America, the Altai region of central Asia and eastern Asia, we consider whether the existing taxonomy based primarily on shell apertural characteristics correlates with relationships established on the basis of mitochondrial and nuclear DNA-sequence data. We obtained DNA sequence from nuclear ITS1 and ITS2 and mitochondrial COI and CytB from 80 specimens across 22 putative Pupilla taxa. The sequence data were analysed using maximum likelihood, maximum parsimony, Bayesian and neighbour-joining phylogenetic tree reconstruction, as well as base-pair substitution and insertion-deletion analysis. Revised species-level concepts were generated by identifying reciprocally monophyletic clades that exhibited unique conchological features. These analyses document that, although many previously described taxa have biological merit, the highly plastic nature of shell apertural features makes them unreliable indicators of species identity in several independent lineages. However, shell surface sculpture and architecture appear to provide more reliable diagnoses. Because of the traditional reliance of species-level taxonomy in Pupilla on plastic apertural features, too many species-level entities have been described in Europe and the Altai. Also, because taxonomically useful shell sculpture features have tended to be ignored, too few species have been described in eastern Asia and North America. As a result, confusion exists about species ranges, ecological tolerances and interpretation of Quaternary fossils within the genus. Based on these analyses three new species are described: P. alaskensis, P. hudsonianum and P. hokkaidoensis. |
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