Introduction of macarpine as a novel cell-permeant DNA dye for live cell imaging and flow cytometry sorting
| Authors | |
|---|---|
| Year of publication | 2016 |
| Type | Article in Periodical |
| Magazine / Source | Biology of the Cell |
| MU Faculty or unit | |
| Citation | |
| Doi | http://dx.doi.org/10.1111/boc.201500047 |
| Field | Genetics and molecular biology |
| Keywords | Cell cycle; DNA dye; FACS; Macarpine; Time-lapse imaging |
| Description | Macarpine (MA) is a quaternary benzophenanthridine plant alkaloid isolated from Macleaya microcarpa or Stylophorum lasiocarpum. Benzophenanthridine alkaloids are interesting natural products that display antiproliferative, antimicrobial, antifungal and anti-inflammatory activities, and also fluorescence properties. In a previous study, we demonstrated that thanks to its ability to interact with DNA and its spectral properties MA could be used as a supravital DNA probe for fluorescence microscopy and flow cytometry including analyses of the cell cycle. In this study, we evaluated the suitability of MA as a DNA dye for time-lapse microscopy and flow-cytometric cell sorting. |
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