HPLC Coupling with ESI, MALDI and SALD ICP MS - The Detection Platform for Metalloprotein Characterization

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Title in English HPLC Coupling with MALDI and SALD ICP MS - The Detection Platform for Metalloprotein Characterization
Authors

JÄGEROVÁ Kateřina BENEŠOVÁ Iva DLABKOVÁ Kristýna KANICKÝ Viktor PREISLER Jan

Year of publication 2016
Type Conference abstract
MU Faculty or unit

Faculty of Science

Citation
Description We demonstrate the use of a detection platform that complements conventional LC-ESI MS with two detection techniques: matrix-assisted laser desorption/ionization (MALDI) and substrate-assisted laser desorption inductively coupled plasma (SALD ICP) mass spectrometry (MS) analyses. As a model system, metallothionein 1, a low molecular protein enabling binding wide range of heavy metals (Zn,Cu,Cd,Hg) was chosen.
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