ERLIC or just HILIC? Investigation of chromatographic behavior of trinucleotides
| Authors | |
|---|---|
| Year of publication | 2016 |
| Type | Article in Proceedings |
| Conference | Advances in chromatography and electrophoresis & Chiranal 2016 |
| MU Faculty or unit | |
| Citation | |
| Field | Analytic chemistry |
| Keywords | HILIC; ERLIC; HPLC; trinucleotides; retention behavior |
| Description | Electrostatic repulsion hydrophilic interaction chromatography (ERLIC) is mixed-mode chromatographic technique which combines to use of electrostatic repulsion and hydrophilic interaction. This chromatographic technique was firstly introduced by J. Alpert in 2008 as an alternative method for separation of highly charged and hydrophilic analytes e.g. peptides, amino acids and nucleotides. Main idea behind ERLIC is based on selective decrease in retention due to repulsion between equally charged stationary phase and analyte. This repulsion effect can be mainly controlled by pH, adjusting presence or absence of charged molecules which result in the isocratic elution of highly retained analytes that would require a gradient in other chromatographic modes such as HILIC or RPLC. |
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