Differentiation between Staphylococcus aureus and Staphylococcus epidermidis strains using Raman spectroscopy

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Authors	REBROŠOVÁ Katarína SILER M. SAMEK O. RŮŽIČKA Filip BERNATOVA S. JEZEK J. ZEMANEK P. HOLÁ Veronika
Year of publication	2017
Type	Article in Periodical
Magazine / Source	FUTURE MICROBIOLOGY
MU Faculty or unit	Faculty of Medicine
Citation
Doi	http://dx.doi.org/10.2217/fmb-2016-0224
Field	Microbiology, virology
Keywords	Raman spectroscopy; rapid diagnostics; Staphylococcus epidermidis; Staphyococcus aureus
Description	Aim: Raman spectroscopy is an analytical method with a broad range of applications across multiple scientific fields. We report on a possibility to differentiate between two important Gram-positive species commonly found in clinical material - Staphylococcus aureus and Staphylococcus epidermidis - using this rapid noninvasive technique. Materials & methods: For this, we tested 87 strains, 41 of S. aureus and 46 of S. epidermidis, directly from colonies grown on a Mueller-Hinton agar plate using Raman spectroscopy. Discussion & conclusion: The method paves a way for separation of these two species even on high number of samples and therefore, it can be potentially used in clinical diagnostics.
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