Characterization of a Porous Nano-electrospray Capillary Emitter at Ultra-low Flow Rates
| Authors | |
|---|---|
| Year of publication | 2017 |
| Type | Article in Periodical |
| Magazine / Source | Journal of Chromatographic Science |
| MU Faculty or unit | |
| Citation | |
| Web | https://academic.oup.com/chromsci/article/55/1/47/2527509 |
| Doi | http://dx.doi.org/10.1093/chromsci/bmw148 |
| Field | Biochemistry |
| Keywords | IONIZATION-MASS-SPECTROMETRY; CE-MS; INTACT PROTEINS; ELECTROPHORESIS; NANOELECTROSPRAY; INTERFACE; ANTIBODIES; TIP; SENSITIVITY; EVAPORATION |
| Description | Biopharmaceuticals, especially therapeutic monoclonal antibodies, have emerged as a very promising new generation of protein-based drugs. However, their comprehensive analysis continues to pose new challenges for the bioanalytical field. Hyphenation of capillary electrophoresis with electrospray ionization (CE-MS) is a promising technique to address these challenges. One of the main advantages of CE-MS is the ability to produce stable electrospray at ultra-low flow rates (5-20 nl/min range). In this short communication we report on the characterization of a porous nano-electrospray capillary emitter focusing on the effects of ultra-low flow rate on ionization efficiency, ion suppression and detection sensitivity. Ion suppression effect of a poorly-ionizable sugar in the presence of an easily-ionized peptide was reduced by almost 2-fold. Intact therapeutic antibody infusion analysis demonstrated that MS detection sensitivity increased by an order of magnitude with the decrease of flow rate from 250 nL/min to 20 nL/min using the nano-electrospray capillary emitter. |
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