EXPRESSION OF REGENERATION-ASSOCIATED PROTEINS IN THE PRIMARY SENSORY NEURONS AND REGENERATING AXONS AFTER NERVE INJURY
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| Year of publication | 2017 |
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| Description | EXPRESSION OF REGENERATION-ASSOCIATED PROTEINS IN THE PRIMARY SENSORY NEURONS AND REGENERATING AXONS AFTER NERVE INJURY Dubový P., Klusáková I., Hradilová-Svíženská I., Brázda V., Joukal M. Department of Anatomy, Cellular and Molecular Research Group, Faculty of Medicine, Masaryk University, Brno, Czech Republic Peripheral nerve injury results in profound alterations of affected neurons involving an interplay between intrinsic and extrinsic molecular events. Restart of regenerative neuronal program is important prerequisite for functional recovery of injured peripheral nerve. The primary sensory neurons with their cell bodies in the dorsal root ganglia (DRG) provide a useful in vivo model to study regulation of neuronal intrinsic regeneration capacity after axotomy. The aim of present experiments was to study quantitative immunodetection of GAP43 and SCG10 as markers of initiation of regeneration program in the rat primary sensory neurons and indicators of axon regeneration in the injured peripheral nerves. We found bilateral increase of GAP43 and SCG10 proteins in different size types of neuronal bodies after unilateral sciatic nerve injury. In addition, DRG sections were double immunostained for GAP43 and SCG10 as well as for the regeneration-associated proteins and molecular markers of neuronal subpopulations (NF200, CGRP, IB4) and activated STAT3. Double immunostaining of GAP43 and SCG10 revealed such differences indicating SCG10 as a better marker of regenerative status of the primary sensory neurons. The better results of regenerated axon detection one day after nerve crush were also obtained using SCG10 than GAP43 immunostaining. Supported by grant 16-08508S of The Czech Science Foundation. |
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