LA-ICP-MS as a sensitive method for detection of nanoparticle-antibody conjugates in immunochemistry analysis
| Authors | |
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| Year of publication | 2019 |
| Type | Article in Proceedings |
| Conference | MENDELNET 2019: PROCEEDINGS OF 26TH INTERNATIONAL PHD STUDENTS CONFERENCE |
| MU Faculty or unit | |
| Citation | |
| Web | https://mendelnet.cz/artkey/mnt-201901-0131_LA-ICP-MS-as-a-sensitive-method-for-detection-of-nanoparticle-antibody-conjugates-in-immunochemistry-analysis.php?back=/magno/mnt/2019/mn1.php?secid=4 |
| Keywords | gold nanoparticles; LA-ICP-MS; immunochemistry; dot-blot; protein detection; p53 |
| Description | Laser ablation followed by inductively coupled plasma mass spectrometry is an analytical method suitable for low concentration measurement of elements in a sample. This can be used not only for detection of elemental distribution within a sample but also as a detection means for immunoanalytical techniques using nanoparticle-antibody conjugates as recognition elements. The aim of this work was to prepare a conjugate of 10nm gold nanoparticles with anti-p53 antibody, to verify the antigen binding ability, and to use this construct for identification of p53 in a real sample of cell lysate. It has been experimentally verified that it is possible to use nanoparticle-modified antibodies for immunochemical analysis coupled to LA-ICP-MS detection. However, the nonspecific sorption of nanoparticles on the sample surface needs to be minimized by optimization of the blocking step. |
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