Epitachophoretic preconcentration of proteins
| Authors | |
|---|---|
| Year of publication | 2021 |
| Type | Article in Proceedings |
| Conference | Proceedings of the 17th International Students Conference “Modern Analytical Chemistry” |
| MU Faculty or unit | |
| Citation | |
| Web | http://web.natur.cuni.cz/analchem/isc/isc17.pdf |
| Keywords | cleaning; epitachophoresis; isotachophoresis; preparative; concentration |
| Description | This project is focused on the epitachophoretic preconcentration of Proteins from biological fluids. In this paper, the method for preparative concentration of three standards of proteins, cytochrome c, myoglobin, and hemoglobin, was developer and described. Concentration conditions, such as concentration and pH of leading and trailing electrolytes, gel composition, and time of concentration, were optimized. The recovery of each protein was at least 70% and concentration factor 50. |
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