Pharmacokinetic profiling via dried blood spot sampling method
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| Year of publication | 2022 |
| Type | Conference abstract |
| Citation | |
| Description | Pharmacokinetic profiles are still an inevitable part of preclinical safety studies. Nowadays, animal models are subjected to ethical standards to be carefully monitored and the principles of the 3Rs are required: replacement, reduction and refinement. Use of dried blood spot (DBS) samples, typically 20-50 µl, rather than the larger blood volumes required to obtain plasma samples, fully embraces the latter two principles of reduction and refinement. We optimized and validated RP-HPLC method (Kinetex C18 column, 100x3.0 mm, 2.6 µm, Phenomenex) hyphenated to mass spectrometry (maXis impact qTOF, Bruker Daltonics) for the determination of two antipsychotics (namely olanzapine and clozapine) and an antidepressant (mirtazapine) in rat DBS samples. The developed LC-MS method offers short analysis cycle time (13 min) with satisfactory sensitivity covering concentration range expected for a pharmacokinetic profile at chosen drug dose. DBS samples for all time points were acquired from just one body, quantified and resultant profiles were figured. |
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