Pharmacokinetic profiling via dried blood spot sampling method
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| Year of publication | 2022 |
| Type | Appeared in Conference without Proceedings |
| Citation | |
| Description | Pharmacokinetic (PK) profiles are still an inevitable part of preclinical safety studies. Generally, PK studies require the collection of a series of blood samples in sufficient volumes (usually about 200 µl). Typically, animal protocols limit the total blood draw between 10 and 15 % of the total blood volume allowed within a 2-week period. Further, animal models are subjected to ethical standards to be carefully monitored and principles of the 3Rs are required: replacement, reduction and refinement. Our pilot PK study of clozapine (CLO) was conducted in a single Wistar albino rat. CLO was administrated subcutaneously in a single dose bolus (20 mg/kg) and blood samples were taken from a retroorbital plexus by a calibrated glass capillary and spotted onto the card (Whatman 903 Protein Saver Card). CLO concentrations were acquired by LC-MS method and corresponding PK parameters were calculated by Kinetica 4.4 software. The obtained parameters are in agreement with data from a large cohort study. |
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