Staining Tissues with Basic Blue 7: A New Dual-Polarity Matrix for MALDI Mass Spectrometry Imaging

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Authors	JAVOREK Michal HENDRYCH Michal ONDRÁKOVÁ Kateřina PREISLER Jan BEDNAŘÍK Antonín
Year of publication	2025
Type	Article in Periodical
Magazine / Source	ANALYTICAL CHEMISTRY
MU Faculty or unit	Faculty of Science
Citation
web	https://pubs.acs.org/doi/10.1021/acs.analchem.4c05244
Doi	http://dx.doi.org/10.1021/acs.analchem.4c05244
Keywords	MALDI matrix; Basic Blue 7; dye; staining; mass spectrometry imaging; MALDI MSI; sample preparation; lipids
Description	A new dye-based matrix (Basic Blue 7) for MALDI mass spectrometry imaging has been discovered. Basic Blue 7 allows a new method of application on samples – tissue staining by sample immersion in dye solution. This protocol allows to record high-quality MALDI MS images in both positive and negative modes, comparable to those obtained with classical matrices 2,5-dihydroxybenzoic acid and 1,5-diaminonaphthalene.
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