MoaB2, a newly identified transcription factor, binds to σA in Mycobacterium smegmatis
| Authors | |
|---|---|
| Year of publication | 2024 |
| Type | Article in Periodical |
| Magazine / Source | Journal of Bacteriology |
| MU Faculty or unit | |
| Citation | |
| web | https://doi.org/10.1128/jb.00066-24 |
| Doi | http://dx.doi.org/10.1128/jb.00066-24 |
| Keywords | MoaB2; sigma(A); mycobacteria; RNA polymerase; transcription |
| Attached files | |
| Description | In mycobacteria, ?A is the primary sigma factor. This essential protein binds to RNA polymerase (RNAP) and mediates transcription initiation of housekeeping genes. Our knowledge about this factor in mycobacteria is limited. Here, we performed an unbiased search for interacting partners of Mycobacterium smegmatis ?A. The search revealed a number of proteins; prominent among them was MoaB2. The ?A-MoaB2 interaction was validated and characterized by several approaches, revealing that it likely does not require RNAP and is specific, as alternative ? factors (e.g., closely related ?B) do not interact with MoaB2. The structure of MoaB2 was solved by X-ray crystallography. By immunoprecipitation and nuclear magnetic resonance, the unique, unstructured N-terminal domain of ?A was identified to play a role in the ?A-MoaB2 interaction. Functional experiments then showed that MoaB2 inhibits ?A-dependent (but not ?B-dependent) transcription and may increase the stability of ?A in the cell. We propose that MoaB2, by sequestering ?A, has a potential to modulate gene expression. In summary, this study has uncovered a new binding partner of mycobacterial ?A, paving the way for future investigation of this phenomenon. |
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