Automated Sequential Derivatization for Gas Chromatography-[Orbitrap] Mass Spectrometry-based Metabolite Profiling of Human Blood-based Samples
| Authors | |
|---|---|
| Year of publication | 2025 |
| Type | Article in Periodical |
| Magazine / Source | Bio-protocol |
| MU Faculty or unit | |
| Citation | |
| web | https://bio-protocol.org/en/bpdetail?id=5196&type=0 |
| Doi | http://dx.doi.org/10.21769/BioProtoc.5196 |
| Keywords | Automation; Derivatization; Thermo ScientificTM TriPlusTM RSH; Metabolite profiling; Gas chromatography-mass spectrometry (GC-MS) |
| Attached files | |
| Description | Many small molecules require derivatization to increase their volatility and to be amenable to gas chromatographic (GC) separation. Derivatization is usually time-consuming, and typical batch-wise procedures increase sample variability. Sequential automation of derivatization via robotic liquid handling enables the overlapping of sample preparation and analysis, maximizing time efficiency and minimizing variability. Herein, a protocol for the fully automated, two-stage derivatization of human blood-based samples in line with GC- [Orbitrap] mass spectrometry (MS)-based metabolomics is described. The protocol delivers a sample-to-sample runtime of 31 min, being suitable for better throughput routine metabolomic analysis. |
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