Fatty Acids Bind to the Fungal Elicitor Cryptogein and Competewith Sterols

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Authors	OSMAN Hanan MIKEŠ Vladimír MILAT Marie Louise PONCHET Michel
Year of publication	2001
Type	Article in Periodical
Magazine / Source	FEBS Letters
MU Faculty or unit	Faculty of Science
Citation
Field	Biochemistry
Keywords	elicitor; erogsterol; elicitin; Phytophthora
Description	Cryptogein is a proteinaceous elicitor of plant defensereactions which also exhibits sterol carrier properties. Inthis study, we report that this protein binds fatty acids. Thestoichiometry of the fatty acid-cryptogein complex is 1:1.Linoleic acid and dehydroergosterol compete for the same site,but elicitin affinity is 27 times lower for fatty acid than farsterol. We show that C7 to C12 saturated and C16 to C22unsaturated fatty acids are the best ligands. The presence ofdouble bonds markedly increases the affinity of cryptogein forfatty acids. A comparison between elicitins and known lipidtransfer proteins is discussed.
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