Stanovení biologicky aktivních látek v léčivých rostlinách pomocí kapilární elektrochromatografie
| Title in English | Determination of Biologically Active Compounds in Medicinal Plants by Capillary Electrochromatography |
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| Authors | |
| Year of publication | 2001 |
| Type | Article in Proceedings |
| Conference | Sborník abstrakt 5. setkání biochemiků a molekulárních biologů |
| MU Faculty or unit | |
| Citation | |
| Field | Biochemistry |
| Keywords | Medicinal Plants; Capillary electrochromatography |
| Description | Capillary electrochromatography (CEC) is a hybrid separation technique that combines the benefits of both capillary zone electrophoresis and high performance liquid chromatography. The high efficiency and resolution of CEC offers an alternative to pressure-driven chromatography systems. Polymer based monolithic capillary columns quickly become a well-established stationary phases in CEC as they eliminated the need of frits and packing procedures. The large variety of available chemistries make these columns an attractive alternative to columns packed with particulate materials. The macroporous polyacrylamide columns for CEC were prepared by in situ copolymerisation of acrylamide, bisacrylamide, vinylsulfonic acid and lauryl acrylate in presence of polyethylene glycol as a porogenic agent. The columns were successfully used to analyse and quantify lignans in extract of the seeds of Schisandra chinensis. Good separations were achieved in less than 20 min. The migration time and peak area reproducibilities were better than 4 %. The results were compared with those obtained by reverse-phase HPLC, the levels of schizandrin, gomisin A, gomisin N and wuweizisu C determined by CEC were in good agreement with those determined by HPLC. |
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