Developmental Consequences of ipt Activation in Germinating Tobacco Seedlings

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Authors	BRZOBOHATÝ Břetislav GENKOV Todor Nikolaev MOORE Ian DUBOVÁ Jaroslava NEJEDLÁ Eliška MALBECK Jiří VÁGNER Martin
Year of publication	2001
Type	Article in Proceedings
Conference	17th International Conference on Plant Growth Substances, Brno, Czech Republic, July 1-6, 2001, Abstracts
MU Faculty or unit	Faculty of Science
Citation
Field	Genetics and molecular biology
Keywords	transcription activation system; isopentenyl transferase; cytokinins; tobacco; Arabidopsis
Description	A transcription activation system based on a synthetic promoter pOp and a corresponding chimeric transcription activator LhG4 was employed to achieve regulatable expression of isopentenyl transferase, IPT, in transgenic tobacco. The system allowed us to analyze in detail developmental consequences of ipt activation in germination and early seedling development. Here we present analysis of (i) dynamics of changes in cytokinin metabolism upon ipt activation and (ii) subsequent chain of developmental alterations leading to transition from originally almost normal seedling to a completely distinct teratoma structure. Major differences in tobacco and Arabidopsis response to ipt activation during germination will be discussed.
Related projects:	Transkription activation system for studying the relationship between cytokinin metabolism and action in Arabidopsis and tobacco