Stanovení glutathion-S-transferasy na pevných elektrodách
| Title in English | Determination of glutathion-S-transferase on solid electrodes |
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| Authors | |
| Year of publication | 2002 |
| Type | Article in Proceedings |
| Conference | Sborník abstrakt III. pracovního setkání fyzikálních chemiků a elektrochemiků v Brně |
| MU Faculty or unit | |
| Citation | |
| Field | Electrochemistry |
| Keywords | glutathion-S-transferasa;pevné elektrody;adsorptivní přenosová diferenční pulzní voltametrie;katalytické procesy bílkovin |
| Description | Glutathion-S-transferase (EC 2.5.1.18 - GST) belongs to the family of enzymes catalysing the connection of tripeptide of glutathione (g-glutamylcysteinyl-glycine) with endogene and exogene substrates. The aim of this paper was to find biosenzor detecting GST in urea. GST (25 kDa) protein from Schizosotoma japonica was purified by chromatography (FPLC Pharmacia ) and electrochemically analyzed on AUTOLAB. Used electrode system was: the working electrode - paste carbon or pyrolytic graphite electrodes (PGE), or hanging mercury dropping electrode (HMDE; the reference elektrode was Ag/AgCl/3M KCl; the auxiliery electrode was platinum wire. In adsorptive transfer differential pulse voltammetry (AdT DPV) GST was adsorbed at the working electrode from 6 mL of the sample. Carbon and graphite electrodes give good reproducible oxidation signals of tryptofane a tyrosine. On HMDE catalytic GST signals were observed (peak H or Brdicka signal). In the range of 5 mg/mL - 100 mg/mL the linear concentration dependence of GST was measured. |
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