Ergosterol induces mobilization of internal calcium in tobacco cells

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Authors	KAŠPAROVSKÝ Tomáš MILAT ML BLEIN JP MIKEŠ Vladimír
Year of publication	2002
Type	Article in Proceedings
Conference	Proc. 6th Conf. EFPP, Prague. Plant Protect. Sci
MU Faculty or unit	Faculty of Science
Citation
Field	Biochemistry
Keywords	ergposterol; plant protection; tobacco; elicitation
Description	Ergosterol interacts with tobacco suspension cells and triggers pH changes of extracellular medium, oxidative burst and synthesis of phytoalexins. Cryptogein stimulated an apparent continuous uptake of external calcium within 40 min, whereas no net uptake of external calcium occurred upon the addition of ergosterol. However, the elicitation with both cryptogein and ergosterol resulted in an increase of the fluorescence of calcium green 1 in cytosol. The use of several inhibitors of calcium channels (La3+, TMB-8, verapamil, ruthenium red, nifedipine) and a protein-kinase inhibitor (staurosporin) suggests that the elicitation with ergosterol includes the mobilization of internal calcium stores in vacuoles mediated by IP3 and serine/threonine protein kinases.
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