Utilisation of nonenzymatic hydrolysis of DNAs for characterisation of extrachromosomal elements in different strains of Bifidobacteria

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Authors	ŠPANOVÁ Alena RITTICH Bohuslav
Year of publication	2002
Type	Article in Proceedings
Conference	Seventh Symposium on Lactic Acid Bacteria
MU Faculty or unit	Faculty of Science
Citation
Field	Microbiology, virology
Keywords	Nonenzymatic hydrolysis; extrachromosomal elements; Bifidobacteria
Description	The nonenzymatic DNA hydrolysis of plasmid DNA in the presence of Eu3+ and La3+ ions can lead to single- and double-stranded breaks in DNA. The cleaved plasmid products can be easily separated and characterised by agarose gel electrophoresis. The nonenzymatic DNA cleavage was used in characterisation of extrachromosomal elements of bacteria of the genus Bifidobacterium.
Related projects:	The use of magnetic carriers with immobilized selective ligands in DNA diagnostics Development of methods for evaluation and rapid identification of selected lactic acid bacteria using molecular biology methods