Determination of purins, pyrimidines and their nucleosides in urine by HPLC and MALDI-TOF mass spectrometry

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Authors	HAVLIŠ Jan REVILLA VÁZQUEZ Alma Luísa KOZÁK Libor HAVEL Josef
Year of publication	1999
Type	R&D Presentation
MU Faculty or unit	Faculty of Science
Citation
Description	Experimental design combined with artificial neural networks enables faster optimization of the separation conditions than classical single variable approach. HPLC can be successfully used for the determination of the state of 2-deoxycytidine 5-monophosphate methylation in DNA. Utilization of ED-ANN within HPLC has greatly enhanced the method of quantitative determination of 5-methyl-2'-deoxycytidine 5'-monophosphate and 2'-deoxycytidine 5'-monophosphate in the cell homogenate. Use of this approach has allowed us to optimize separation of several types of mixtures containing purine and pyrimidine bases, their nucleosides and nucleotides.
Related projects:	Structure and character of bonding, properties and analysis of synthetic and natural molecular ensembles