Analýza proteomu bakterie Paracoccus denitrificans

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Title in English	Analysis of proteome of bacterium Paracoccus denitrificans
Authors	BOUCHAL Pavel PŘECECHTĚLOVÁ Petra ZDRÁHAL Zbyněk KUČERA Igor
Year of publication	2003
Type	Article in Proceedings
Conference	VII. Pracovní setkání biochemiků a molekulárních biologů. Sborník příspěvků
MU Faculty or unit	Faculty of Science
Citation
Field	Biochemistry
Keywords	Proteome; Paracoccus denitrificans; Membrane proteins; Two-dimensional electrophoresis; Mass spectrometry
Description	Gramnegative bacterium Paracoccus denitrificans is able to grow under broad variety of conditions. Adaptation to altered conditions strongly depends on changes in expression of metabolically active proteins. For study on changes of protein levels in whole-cell extract as well as in bacterial membrane of Paracoccus denitrificans, we have developed methods based on two-dimensional electrophoresis with immobilised pH gradients. Optimization of critical points of both protocols is demonstrated. We have compared effect of anaerobiosis on protein composition of bacterial membrane using optimized method. Several proteins on protein maps were sucessfully identified using MALDI-TOF mass spectrometry.
Related projects:	Mechanisms involved in regulation of respiratory systems in denitrification bacteria by environmental factors