Identifikace bakterií druhu Bifidobacterium longum pomocí polymerázové řetězové reakce a restrikční analýzy amplifikované ribozomální DNA (ARDRA)

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Title in English	Identification of Bifidobacterium longum by polymerase chain reaction and amplified ribosomal DNA restriction analysis
Authors	KŘÍŽOVÁ Jana ŠPANOVÁ Alena RITTICH Bohuslav
Year of publication	2003
Type	Article in Proceedings
Conference	VII. Pracovní setkání biochemiků a molekulárních biologů
MU Faculty or unit	Faculty of Science
Citation
Field	Microbiology, virology
Keywords	Bifidobacterium longum; identification; ARDRA
Description	Method ARDR is based on amplification of DNA region coding 16S rRNA. Genus Bifidobacterium specific amplicon of the length of 914 bp is amplified with the use of primers PbiF1 and PBiR2. This genus specific amplicon was used for restriction analysis. This procedure can be used for resolution of species B.infantis, B. longum and B. animalis. Using PCR with species specific primers was identified the species B. longum.
Related projects:	Development of methods for evaluation and rapid identification of selected lactic acid bacteria using molecular biology methods