Quantification of proteins by means of spectrophotometry. electrophoresis and electrochemistry

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Title in English	Quantification of proteins by means of spectrophotometry, electrophoresis and electrochemistry
Authors	KIZEK René VACEK Jan BRÁZDOVÁ Marie TRNKOVÁ Libuše
Year of publication	2003
Type	Article in Proceedings
Conference	Sborník příspěvků, VII. Pracovní setkání biochemiků a molekulárních biologů
MU Faculty or unit	Faculty of Science
Citation
Field	Analytic chemistry
Keywords	spectrophotometry;UV-Vis spectra;electrophoresis;PAA gel;electrochemistry;DPV and CPSA;proteins;detection limit
Description	For the determination of proteins, a lot of methods can be used. It is suitable to follow some criteria: (a) amount of proteins being available for analysis, (b)concentartion of proteins in samples, (c)specificity of method, and (d) facility and confidence of chosen method. In addition to spectrophotometric and electrophoretic methods, electrochemistral methods (differential pulse voltammetry and chronopotentometric stripping analysis) have been applied to the determination of bovine serum albumin - BSA, Mr 67 kDa)and tumor supressor p 53 (Mr 43 kDa). The results using these methods have been compared.
Related projects:	Structure and character of bonding, properties and analysis of synthetic and natural molecular ensembles