Application of avidin-biotin technology and adsorptive transfer stripping square-wave voltammetry for detection of DNA hybridization and avidin in transgenic avidin maize

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Authors	MASAŘÍK Michal KIZEK René KRAMER K.J. BILLOVÁ Sabina BRÁZDOVÁ Marie VACEK Jan BAILEY Michele JELEN František HOWARD A. J.
Year of publication	2003
Type	Article in Periodical
Magazine / Source	Analytical Chemistry
MU Faculty or unit	Faculty of Science
Citation
Field	Genetics and molecular biology
Keywords	Streptavidin; Avidin; Adorptive Transfer Stripping Voltammetry; Square-Wave Voltammetry; Biotin Binding; Transgenic Avidin Maize; Corn; Grain; Transgenic Protein Detection; Electrochemical Detection; DNA Hybridization
Description	The proteins streptavidin and avidin were electrochemically detected in solution by adsorptive transfer stripping square wave voltammetry (AdTS SWV) at a carbon paste electrode (CPE). AdTS SWV was used to quantify biotinylated oligonucleotides, DNA hybridizations, and avidin in extracts of transgenic avidin maize. The detection limits of denatured and native streptavidin were 6 pM and 120 nM, respectively. The results demonstrated that streptavidin/avidin AdTS SWV is a sensitive and specific method for quatifying DNA and proteins in biological samples such as foods and tissue extracts, including genetically modified crops (avidin maize) and other plants in neighboring fields.
Related projects:	New trends in electrochemistry of nucleic acids and their applications in enviromental chemistry Application of adsorptive transfer and elemination techniques in oligonucleotides and nucleic acids analysis.