Hledání rostlinných telomer-vazebných proteinů prostřednictvím genomického a proteomického přístupu
| Title in English | Searching for plant telomere-binding proteins using genomic and proteomic approaches |
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| Authors | |
| Year of publication | 2003 |
| Type | Article in Proceedings |
| Conference | VII. Pracovní setkání biochemiků a molekulárních biologů |
| MU Faculty or unit | |
| Citation | |
| Field | Genetics and molecular biology |
| Keywords | telomere-binding proteins; Arabidopsis thaliana |
| Description | Two proteins, AtTBP2 (acc. nos. T46051 (protein database), GI:638639 (nucleotide database)) and AtTBP3 (BAB08466, GI:9757879) from Arabidopsis thaliana, were found in the database on the basis of amino acid sequence similarity with telomere-binding protein PcMyb1 (acc. No. T15038) from Petroselinum crispum. Both of the proteins were expressed in various A. thaliana tissues and as the PcMyb2 contain Myb-like domain at the N-terminus which enables the double-stranded DNA binding and additionally a histone H1/H5-like DNA-binding domain in the middle of the protein sequence. The cDNAs of ATTB1 and ATTB2 were cloned and the experiments carried out in Saccharomyces cerevisiae using the two-hybrid system showed interaction between the two proteins and self-interaction of each of the proteins. |
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