Capillary Electrophoresis of Bacteriophage 812 Proteins Combined with MALDI Mass Spectrometry

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Authors	PREISLER Jan VRÁBEL Patrik NOVÁKOVÁ Jaromíra SPĚŠNÝ Michal ZDRÁHAL Zbyněk KONEČNÁ Hana KRÁSENSKÝ Pavel EYER Luděk DOŠKAŘ Jiří PANTŮČEK Roman FORET František KLEPÁRNÍK Karel
Year of publication	2004
Type	Conference abstract
MU Faculty or unit	Faculty of Science
Citation
Description	The alarming increase in antibiotic-resistant bacteria has renewed interest in bacteriophage therapy of various human infections, such as postoperative staphylococcal wound infections or anthrax. In this work, SDS gel electrophoresis was used for the protein separation followed by tryptic digestion and ESI or MALDI analysis.Additionally, we have proposed a new approach to the analysis of the phage proteins, especially low abundant proteins, such as lytic enzymes. Protein and peptide separations were performed with capillary electrophoresis (CE) and a new universal interface has been developed to couple CE with MALDI MS. A method of protein microdigestion on MALDI target is being studied and will be used for protein identification.
Related projects:	Integrated genome and proteome analysis of therapeutically important bacteriophages by combination of electrophoresis and mass spectrometry