MIDAZOLAM JAKO SELEKTIVNÍ MARKER PODRODINY 3A CYTOCHROMU P450; FARMAKOKINETICKÁ CHARAKTERISTIKA MIDAZOLAMU
| Title in English | Midazolam as a probe substrate of 3A subfamily CYP-450, pharmacological parameters of midazolam |
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| Authors | |
| Year of publication | 2004 |
| Type | Article in Proceedings |
| Conference | Sborník abstrakt, Medifórum |
| MU Faculty or unit | |
| Citation | |
| Field | Pharmacology and pharmaceutical chemistry |
| Keywords | Midazolam; CYP-450 |
| Description | The aim of the project was to develop HPLC method for determination of midazolam and its three metabolites, 1- and 4-hydroxymidazolam and 1,4-dihydroxymidazolam from perfuse rat liver medium. Hydroxylation of midazolam is used as a valuable tool for measurement of metabolic activity of the 3A1/2 isoform of cytochrome P-450 in rats (CYP3A1/2)This method is simple, rapid, sensitive and applicable for modeling and description of possible pharmacological interactions on rat CYP3A1/2 or it`s human orthologue CYP3A4/5, respectively. In the second part of the project, there was performed pharmacokinetic study after i.v. administration of midazolam. |
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