The  protonation/deprotonation equilibrium of azido-analogues of thymidine  and uridine studied by UV spectroscopy  and chemometrics

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Authors	TRNKOVÁ Libuše TOPINKOVÁ Jana
Year of publication	2005
Type	Article in Proceedings
Conference	sborník přispěvků- V. Pracovní setkání fyzikálních chemiků a elektrochemiků
MU Faculty or unit	Faculty of Science
Citation
Field	Physical chemistry and theoretical chemistry
Keywords	Azido-analogues of thymidine and uridine; UV spektra; chemometrics
Description	In the present work we studied protonation/deprotonation equilibrium of azido-derivatives in aqueous solutions at various ionic strength by using UV spectra and chemometrics. The absorption spectra were measured from 240 to 320 nm at 25C.The absoption maxima of nucleic bases and their derivatives are very close and identical.From non-smoothed spectra the matrices were obtained that were used as input data for Multivariate Curve Resolution including the Alternation Least Squates (MCR-ALS, Matlab).
Related projects:	New trends in electrochemistry of nucleic acids and their applications in enviromental chemistry Application of adsorptive transfer and elemination techniques in oligonucleotides and nucleic acids analysis. Structure and character of bonding, properties and analysis of synthetic and natural molecular ensembles