Matrix Assisted Laser Desorption/Ionization Mass Spectrometry Identification of Fluorescently Labeled Peptides for Optimization of Laser Induced Fluorescence Detection

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Authors	VRÁBEL Patrik TÁBORSKÝ Petr RYVOLOVÁ Markéta HAVEL Josef PREISLER Jan
Year of publication	2004
Type	Article in Proceedings
Conference	Journal of Peptide Science, 3rd International and 28th European PEPTIDE Symposium
MU Faculty or unit	Faculty of Science
Citation
Web	http://www3.interscience.wiley.com/cgi-bin/jissue/109608640
Field	Analytic chemistry
Keywords	electrophoresis; MALDITOF MS; fluorescence; derivatization; rhodamine B isothiocyanate; peptides
Description	Laser induced fluorescence detection (LIF) is an effective for the sensitive determination of biologically important molecules, such as peptides and proteins, after Capillary Electrophoresis (CE) separation. In this work we demonstrate preliminary results obtained using CELIF and matrix assisted laser desorption/ionization mass spectrometry (MALDI MS) to investigate derivatization of model peptides with rhodamine-based reagents.
Related projects:	Integrated genome and proteome analysis of therapeutically important bacteriophages by combination of electrophoresis and mass spectrometry