Využití nosičů s karboxylovými skupinami pro PCR charakterizaci bifidobakterií v mléčných výrobcích

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Title in English	The use of carboxyl coated methacrylate based microspheres for Bifidobacterium spp. PCR detection in dairy products
Authors	KŘÍŽOVÁ Jana ŠPANOVÁ Alena RITTICH Bohuslav
Year of publication	2004
Type	Article in Proceedings
Conference	XIII. konference mladých mikrobiologů: Tomáškovy dny, Brno 2-4.6. 2004
MU Faculty or unit	Faculty of Science
Citation
Field	Microbiology, virology
Keywords	Carboxyl coated methacrylate based microspheres; Bifidobacterium; PCR ; dairy products
Description	The SPRI method using magnetic and nonmagnetic carboxyl coated microspheres was optimalised for isolation and purification of Bifidobacterium spp. DNA from dairy products. The differencies in the amplification of various dairy products were observed.
Related projects:	The use of magnetic carriers with immobilized selective ligands in DNA diagnostics Development of methods for evaluation and rapid identification of selected lactic acid bacteria using molecular biology methods